Associate Scientist, Cell Line Development
Denali Therapeutics
Summary
We are looking for a highly motivated and collaborative scientist to join our Bioprocess Development team, focused on cell line development (CLD). The successful candidate will be joining a multidisciplinary team to generate commercially viable cell lines for novel and complex therapeutic molecules including bispecific antibodies, Fc fusion proteins, and enzyme fusions.
Job description
Key Responsibilities
- Support the development of Denali's mammalian biotherapeutic production platform to generate commercially viable cell lines.
- Execute cell line development activities including transfection, selection, single cell sorting, clone imaging, clone selection, and clone banking for pipeline projects.
- Design, execute, and interpret cell line development experiments in collaboration with other scientists in the group, including the use of JMP statistical software for DOE experiments and generating figures.
- Write technical and summary reports including cell line development reports which serve as source documents for regulatory filings, effectively communicate findings with cross-functional teams, and participate in scientific discussions with scientific teams.
- Maintain accurate and detailed laboratory notebook and relevant documentation.
- Cross-functionally support cell culture process development through bioreactor production runs and DOE-based screening experiments as necessary.
- Evaluate, optimize, and implement characterization assays such qPCR, Western blots, and flow cytometry.
Requirements
- BA/BS or MS degree in Molecular Biology/Biochemistry/Cell Biology or related field
- Technical proficiency in mammalian cell culture and upstream process development preferably in an industrial laboratory
- 2+ years of experience working in a laboratory environment
- Experience in CHO cell line development for biologics in a CMC setting preferred
- Proficiency with protein characterization techniques such as Octet, SDS-PAGE, Caliper, and HPLC
- Demonstrated ability to organize, analyze, and interpret experimental data, specifically in JMP and Excel
- Ability to work effectively and collaboratively with other scientists in cross-functional project teams
- Excellent oral presentation and written communication skills
Requirements
- BA/BS or MS degree in Molecular Biology/Biochemistry/Cell Biology or related field
- Technical proficiency in mammalian cell culture and upstream process development preferably in an industrial laboratory
- 2+ years of experience working in a laboratory environment
- Experience in CHO cell line development for biologics in a CMC setting preferred
- Proficiency with protein characterization techniques such as Octet, SDS-PAGE, Caliper, and HPLC
- Demonstrated ability to organize, analyze, and interpret experimental data, specifically in JMP and Excel
- Ability to work effectively and collaboratively with other scientists in cross-functional project teams
- Excellent oral presentation and written communication skills
Benefits
- 401(k)
- Healthcare coverage
- ESPP
Company overview
Denali Therapeutics is a pioneering biotechnology company focused on developing innovative therapeutics for neurodegenerative and lysosomal storage diseases. Founded in 2015 and headquartered in South San Francisco, California, the company has developed a proprietary TransportVehicle™ (TV) platform designed to enable biotherapeutics to cross the blood-brain barrier, addressing one of the most significant challenges in treating central nervous system disorders. Denali's pipeline includes promising candidates for conditions such as Hunter syndrome (MPS II), Parkinson's disease, frontotemporal dementia, Sanfilippo syndrome (MPS IIIA), and MPS I, with several programs in advanced clinical development stages. The company collaborates with major pharmaceutical partners including Biogen and Sanofi, leveraging its platform technology to create a new class of barrier-crossing therapeutics. With approximately 500-1,000 employees and operations spanning multiple locations including Salt Lake City, Denali Therapeutics represents a leading force in neuroscience drug development, combining deep scientific expertise with a patient-centric approach to transform treatment paradigms for serious neurological conditions.