Associate Scientist, Protein Sciences
Septerna
Summary
We are seeking passionate and creative individuals who want to contribute to Septerna's mission to utilize innovative drug discovery technologies for GPCR targets. The ideal candidate will have experience in eukaryotic cell culture of suspension-based cell lines including HEK293, including cell line maintenance, transfection, and protein expression.
Job description
About The Role
We are seeking passionate and creative individuals who want to contribute to Septerna's mission to utilize innovative drug discovery technologies for GPCR targets. The ideal candidate will have experience in eukaryotic cell culture of suspension-based cell lines including HEK293, including cell line maintenance, transfection, and protein expression.
The chosen candidate will be working in a collaborative team contributing to improvements in protein expression and purification to support production and characterization of proteins for use in structural biology, biophysics, and small molecule drug discovery.
Responsibilities
- Perform as a hands-on cell culturist to help fully enable Septerna's Native Complex
- Carry out continuous cell line maintenance primarily on Expi293 suspension cell lines, but also of insect cell lines when required
- Perform transient transfections, stable pool selection, and cell line cryopreservation of Expi293 cell lines
- Carry out small- and large-scale cell culture for production of recombinant proteins
- Assist when needed with protein purification and protein-related assay technologies including ELISAs, SDS-PAGE, and DSF
- Work collaboratively with Discovery Biology teams in ensuring proper prioritization and timelines of cell culture requests
- Laboratory notebooking, data analysis and documentation
Qualifications
- BS/MS with a minimum of 4+ years of hands-on relevant experience in a biotechnology or pharmaceutical setting
- 1+ years demonstrated expertise in high-quality mammalian cell culture of suspension cell lines including HEK293, preferably using the Expi293 system
- Experience in transient transfection, cell line maintenance, cell counting, stable pool selection, and cell cryopreservation
- Prior experience with ELISA assays and basic protein techniques (SDS-PAGE, Nanodrop, DSF) is desirable
- Ability to organize multiple projects to ensure delivery of reagents with appropriate prioritization and timelines
- Demonstrated experience working collaboratively in a team-oriented environment
- Clear and effective verbal and written communication skills
Requirements
- BS/MS with a minimum of 4+ years of hands-on relevant experience in a biotechnology or pharmaceutical setting
- 1+ years demonstrated expertise in high-quality mammalian cell culture of suspension cell lines including HEK293, preferably using the Expi293 system
- Experience in transient transfection, cell line maintenance, cell counting, stable pool selection, and cell cryopreservation
- Prior experience with ELISA assays and basic protein techniques (SDS-PAGE, Nanodrop, DSF) is desirable
- Ability to organize multiple projects to ensure delivery of reagents with appropriate prioritization and timelines
- Demonstrated experience working collaboratively in a team-oriented environment
- Clear and effective verbal and written communication skills
Benefits
- Benefits
- Stock options
- Annual target bonus
Company overview
Septerna is an innovative biotechnology company specializing in the discovery and development of novel therapeutics targeting G-protein coupled receptors (GPCRs). Founded by scientific leaders in the GPCR field, the company leverages proprietary Native Complex™ platform technology to unlock previously inaccessible GPCR targets. Septerna's approach enables the identification of small molecule drugs for historically challenging GPCRs, which represent one of the largest and most important classes of drug targets in human biology. The company focuses on developing treatments for a wide range of diseases including metabolic disorders, endocrine conditions, and other therapeutic areas where GPCR modulation can provide significant clinical benefits. With its cutting-edge technology platform and experienced team, Septerna aims to advance a pipeline of first-in-class and best-in-class therapeutics that address unmet medical needs through novel mechanisms of action.