Biological Scientist

This position designs, executes, and optimizes advanced molecular, cellular, genomic, and functional studies using genome engineering, stem cell biology, high-throughput screening, imaging, sequencing, and specialized analytical platforms to support mechanistic and translational research. The role also provides scientific and technical leadership in laboratory operations, quality control, instrumentation, data analysis, documentation, and multidisciplinary collaboration, contributing to research programs, publications, presentations, and grant-related activities.

Responsibilities

• Design, execute, and optimize molecular biology and cell-based experiments utilizing advanced techniques including CRISPR genome editing, cloning, construct design, gene expression analysis, and functional genomics approaches.
• Prepare and process samples for next-generation sequencing (NGS), transcriptomic analyses, single-cell sequencing, PCR, qPCR, and other genomic applications, including library preparation and QC.
• Conduct transcriptomic and genomic data generation workflows, including sample preparation, sequencing support, and coordination with bioinformatics teams for downstream analyses.
• Coordinate all aspects of cell banking and cell line stewardship, including cryopreservation, recovery, authentication, distribution, shipment and receipt of cell lines, and maintenance of associated records.
• Collaborate with multidisciplinary research teams, contributing scientific expertise in molecular biology, genome engineering, stem cell biology, assay development, and translational research to support program objectives and publications.
• Perform and optimize mammalian cell culture experiments, including maintenance of primary cells, stem cells, immortalized cell lines, and differentiated cell populations under sterile conditions.
• Differentiate stem cells into specialized cell types using established and novel protocols, automated culture systems, and high throughput platforms to support translational and mechanistic studies.
• Design and generate genetic constructs through molecular cloning, plasmid engineering, CRISPR/Cas-based genome editing, and viral vector preparation to support functional studies.
• Isolate, quantify, and assess quality of DNA, RNA, and protein samples from cultured cells and tissue specimens using manual and automated workflows.
• Perform protein characterization assays, including Western blotting, immunocytochemistry, immunofluorescence staining, and related molecular and cellular analyses.
• Prepare and analyze tissue specimens using cryostat sectioning, microscopy, and histological techniques, supporting cellular and molecular characterization studies.
• Develop, validate, and implement high-throughput screening assays, including bioluminescence-, fluorescence-, and reporter-based assays for drug discovery and functional genomics applications.
• Perform cellular functional assays using instrumentation such as Seahorse metabolic flux analyzers, multi-electrode arrays, flow cytometers, and other specialized research platforms.
• Coordinate cell line QC programs, including mycoplasma testing, karyotyping, STR/DNA fingerprinting, contamination monitoring, and validation of genetically modified cell lines.
• Establish, maintain, and update laboratory inventories for reagents, antibodies, plasmids, cell lines, small molecules, consumables, and experimental protocols, ensuring accurate documentation and compliance with laboratory quality standards.
• Operate and maintain advanced imaging systems, including fluorescence and confocal microscopy platforms, and perform quantitative image analysis to assess cellular phenotypes and molecular markers.
• Maintain comprehensive laboratory records, including electronic laboratory notebooks, experimental protocols, standard operating procedures (SOPs), and regulatory documentation to ensure reproducibility and compliance.
• Monitor laboratory instrumentation and infrastructure, including troubleshooting equipment, coordinating maintenance and service contracts, validating instrument performance, and training laboratory personnel on proper operation.
• Analyze, interpret, and present experimental data, prepare technical reports, manuscripts, protocols, and presentations, and contribute to grant applications, scientific publications, and collaborative research initiatives.