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Research Associate I/II - Molecular Cloning

BioLegend

San Diego, CAOnsitePosted 29 days ago$22.00 - $29.50/hour
Full TimeEntry LevelResearch & ScientistLife Science Service & Supply
Original posting ↗

Summary

This position is responsible for developing recombinant protein/antibody research products in the Molecular Cellular Biology group at BioLegend. The research associate will be involved in the early stage of product development for recombinant protein/antibody products.

Job description

Essential Functions

  • Prepare cell culture media for experiments.
  • Perform cloning, e.g. seamless cloning, and verify the plasmid sequence.
  • Perform PCR and DNA purification for molecular cloning.
  • Perform mammalian and bacterial cell cultures.
  • Purify recombinant protein/antibodies by affinity tag.
  • Perform protein SDS-PAGE gel and DNA agarose gel.
  • Documentation of experiments and generation of reports in conjunction with other research staff.
  • Maintain DNA inventory, laboratory equipment, and manage supplies.
  • Assist in troubleshooting of experiments and the development of new laboratory procedures under appropriate supervision.
  • Other projects or responsibilities as may be required.

Requirements

  • Bachelors of Science in Biological Sciences
  • 1 year of general training in molecular biological laboratory or research environment
  • Experience with maintenance of suspension mammalian cell lines culture
  • Research & development experience in any industrial R&D environment
  • Good analytical, communication, and organization skills
  • Demonstrated ability to be detail-oriented, organized and work well in small workgroups
  • Able to perform basic computer operations such as MS Office and Outlook and other computer-based software such as Snapgene

Benefits

  • Medical Insurance
  • Dental Insurance
  • Vision Insurance
  • Life Insurance
  • Disability Insurance
  • Paid Time Off
  • Parental Benefits
  • Compassionate Care Leave
  • 401(k) With Company Match
  • Employee Stock Purchase Plan

Company overview

BioLegend is a global leader in the development and manufacturing of high-quality antibodies, reagents, and research tools for biomedical and life science research. Founded in 2002 and headquartered in San Diego, California, the company has established itself as a premier provider of innovative solutions that enable groundbreaking discoveries across multiple scientific disciplines. As a subsidiary of Revvity (formerly part of PerkinElmer), BioLegend leverages extensive resources to deliver comprehensive product portfolios spanning immunology, neuroscience, cancer research, stem cell biology, and proteogenomics. The company's state-of-the-art 8-acre campus in San Diego accommodates over 500 employees and features advanced manufacturing facilities, including San Diego's first robotic picking system in cold storage. BioLegend's product offerings include flow cytometry antibodies, ELISA kits, recombinant proteins, custom assay development services, and cutting-edge technologies like TotalSeq™ antibody-oligonucleotide conjugates for single-cell proteogenomics. With a commitment to accelerating scientific discovery from research to cure, BioLegend serves researchers worldwide through its global network, supporting advancements in cancer immunotherapy, neurodegenerative diseases, immunology, and other critical areas of biomedical research.

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