Scientist, Cell Engineering
Colossal Biosciences
Summary
An affiliate of Colossal is hiring a Scientist that will derive non-model organism primary cell lines, conduct assays corresponding to cell health, characterize lines, perform transfections, and deliver edited lines for downstream SCNT. The candidate will be part of a core team responsible for optimizing and characterizing for lines derived in house and supporting other parts of the cell core team in gene editing workflows. This position reports to the Manager of Primary Cell and Production sub-team within the Cell Engineering and Production (CEP) core team. This position is based out of Dallas, TX. Relocation assistance is available.
Job description
Duties and Responsibilities
- Isolate, expand, and maintain high-quality primary cell cultures from diverse non-model animal tissues.
- Innovate then execute experiments to generate and characterize primary cells from non-model mammalian organisms, develop functional assays, assess and interpret experimental workflows
- Work with team members to assess efficiency of workflows through cellular assays and NGS sequencing approaches
- Work closely with genome engineers to screen, validate, and make cell lines that will undergo SCNT
- Work on project milestones
- Attend lab meetings, prepare reports and presentations on the progress of projects
- Great organizational skills and excellent laboratory record maintenance
Required Skills and Abilities
- Expert knowledge of primary cell derivation, culture, propagation, and biobanking
- Expert knowledge and hands-on experience in CRISPR gene editing, including proficiency with multiple gene delivery methods such as chemical transfection, electroporation, and LNP delivery.
- Experience with flow cytometry, immunostaining, confocal microscopy
- Microdissection experience
- Strong Aseptic Technique and ability to work with antibiotic free culture
- Strong written and oral communication skills, as well as creative problem-solving skills
- Enjoys the fast-paced startup environment and teamwork
- Exceptional organizational skills, multitasking, and attention to detail.
- Strong emotional intelligence and passion for building relationships across all levels.
- Strong analytical and problem-solving skills.
- Ability to function well in a growth-oriented, nimble environment.
Preferred Experience
- Experience with automated cell culture workflows.
- Previous work with mESC editing pipelines
Required Education and Experience
- PhD in biological sciences, stem cell biology, genetics or related field. BS/MS with significant relevant experience will also be considered
- 4+ years hands-on expertise in mammalian cell culture systems
Requirements
- Expert knowledge of primary cell derivation, culture, propagation, and biobanking
- Expert knowledge and hands-on experience in CRISPR gene editing, including proficiency with multiple gene delivery methods such as chemical transfection, electroporation, and LNP delivery.
- Experience with flow cytometry, immunostaining, confocal microscopy
- Microdissection experience
- Strong Aseptic Technique and ability to work with antibiotic free culture
- Strong written and oral communication skills, as well as creative problem-solving skills
- Enjoys the fast-paced startup environment and teamwork
- Exceptional organizational skills, multitasking, and attention to detail.
- Strong emotional intelligence and passion for building relationships across all levels.
- Strong analytical and problem-solving skills.
- Ability to function well in a growth-oriented, nimble environment.
- PhD in biological sciences, stem cell biology, genetics or related field. BS/MS with significant relevant experience will also be considered
- 4+ years hands-on expertise in mammalian cell culture systems
Benefits
- Medical, Dental & Vision
- Paid Time Off
- Company Holidays
- Flexible Spending Accounts
- 401(k) Matching
- Paid Parental Leave
- Education Reimbursement
Company overview
Colossal Biosciences is a pioneering American biotechnology and genetic engineering company founded in 2021 by Harvard geneticist George Church and serial entrepreneur Ben Lamm. Headquartered in Dallas, Texas, the company specializes in developing radical new technologies to advance the field of genomics and species restoration. Colossal is the first company to apply CRISPR gene-editing technology for the purpose of species de-extinction, beginning with ambitious projects to revive the woolly mammoth, Tasmanian tiger, dodo bird, dire wolf, and other extinct species. The company's mission extends beyond de-extinction to include protection of critically endangered species and repopulation of critical ecosystems that support biodiversity and ecosystem health. With over 170 scientists and advisors, Colossal combines cutting-edge genetic engineering with conservation biology to address the global biodiversity crisis. The company has raised significant funding, achieving unicorn status and becoming Texas' first decacorn with a valuation exceeding $10 billion. Beyond its scientific endeavors, Colossal has spun out software platforms like Form Bio and established the Colossal Foundation to support conservation efforts worldwide.