Summary

Nona Biosciences is a global biotechnology company headquartered in Cambridge, Massachusetts, specializing in fully human antibody-based therapeutic discovery, engineering, and development. Founded in 2022 as a wholly-owned subsidiary of HBM Holdings Limited, the company leverages its proprietary Harbour Mice® platform to generate fully human monoclonal antibodies in both traditional two heavy and two light chain (H2L2) format and heavy chain only (HCAb) format. Nona Biosciences provides integrated antibody discovery services ranging from antigen preparation and animal immunization to antibody lead generation, engineering, developability assessment, and pharmacological evaluation. The company's comprehensive "Idea to IND" (I to I®) solution spans target validation through preclinical research, serving academic institutions, biotech startups, and biopharmaceutical giants worldwide. With over 50 industry and academic partners across more than 200 projects, Nona Biosciences has established itself as a trusted partner in driving global inventions of transformative next-generation drugs through diversified partnership strategies including co-discovery, platform licensing, and antibody discovery collaborations.

Job description

Position Summary: Scientist II ~ Senior Scientist II, In Vivo

Responsibilities

Help to plan and execute mouse in vivo studies including immunizations, titer check and efficacy testing
Lead antibody discovery efforts utilizing single B-cell screening technology
Execute mammalian cell transfections, lentiviral vector production, and cell transductions
Design and perform flow cytometry assays for target binding, immunophenotyping, and functional evaluation of immune cells
Conduct ELISA and MSD analyses to assess antibody responses in immunized animals and quantify cytokine production in primary cell-based assays
Perform in vitro assays, including primary human immune cell isolation, culture and differentiation
Conduct independent data analysis contributing to scientific discussion and presentations
Collaborate with other team members to support internal and external projects

Requirements

Master’s or Ph.D. degree in Biology or a related scientific discipline
Experience with single-cell sequencing, particularly for antibody discovery, is highly preferred
3+ years of hands-on experience with mammalian cell culture and molecular biology
3+ years of hands-on experience working with mice, including euthanasia and tissue collection
Experience with or knowledge of flow cytometry and ELISA is highly desired
Excellent attention to detail, strong documentation skills, and effective written and verbal communication skills
Strong organizational and planning skills, with the ability to meet deadlines and manage multiple tasks simultaneously
Ability to quickly learn new scientific concepts and experimental techniques
Diligent, self-motivated, independent, and highly responsible
Strong interpersonal and communication skills, with the ability to build effective relationships across internal functions and with external partners

Requirements

Master’s or Ph.D. degree in Biology or a related scientific discipline
Experience with single-cell sequencing, particularly for antibody discovery, is highly preferred
3+ years of hands-on experience with mammalian cell culture and molecular biology
3+ years of hands-on experience working with mice, including euthanasia and tissue collection
Experience with or knowledge of flow cytometry and ELISA is highly desired
Excellent attention to detail, strong documentation skills, and effective written and verbal communication skills
Strong organizational and planning skills, with the ability to meet deadlines and manage multiple tasks simultaneously
Ability to quickly learn new scientific concepts and experimental techniques
Diligent, self-motivated, independent, and highly responsible
Strong interpersonal and communication skills, with the ability to build effective relationships across internal functions and with external partners

Company overview

Nona Biosciences is a global biotechnology company headquartered in Cambridge, Massachusetts, specializing in fully human antibody-based therapeutic discovery, engineering, and development. Founded in 2022 as a wholly-owned subsidiary of HBM Holdings Limited, the company leverages its proprietary Harbour Mice® platform to generate fully human monoclonal antibodies in both traditional two heavy and two light chain (H2L2) format and heavy chain only (HCAb) format. Nona Biosciences provides integrated antibody discovery services ranging from antigen preparation and animal immunization to antibody lead generation, engineering, developability assessment, and pharmacological evaluation. The company's comprehensive "Idea to IND" (I to I®) solution spans target validation through preclinical research, serving academic institutions, biotech startups, and biopharmaceutical giants worldwide. With over 50 industry and academic partners across more than 200 projects, Nona Biosciences has established itself as a trusted partner in driving global inventions of transformative next-generation drugs through diversified partnership strategies including co-discovery, platform licensing, and antibody discovery collaborations.